Two New Cryptic Endemic Toads of Bufo Discovered in Central Nevada, Western United States (Amphibia: Bufonidae: Bufo [Anaxyrus])
Michelle R. Gordon, Eric T. Simandle, Franziska C. Sandmeier, and C. Richard Tracy
Copeia 108: 166–183
Figure S1. Major and minor groups identified: Bayesian inference phylogenetic tree constructed from analyses from unique haplotype sequences of 1,622 bp fragment of the control region of the mitochondrial genome (Fig. 1B; n = 308). Posterior probabilities are shown. Haplotype number (n = 62) and sampling locality comprise terminal ends of tree and two haplotypes of the root are shown. Scale bar represents substitutions per site. Minor groups include localized species: B. nelsoni (green), B. exsul (orange), B. canorus (purple), B. williamsi (red), and newly delimited species, B. monfontanus (mauve) and B. nevadensis (pink) of the Eastern Great Basin clade. Large bars identify major groups (S–Mojave; HL–Humboldt-Lahontan; W–Western Great Basin), which include populations of B. boreas, sampled within the hydrological Great Basin (Fig. 1B). Analyses conducted in Mr. Bayes v3.1.2 (Ronquist and Huelsenbeck, 2003).
Figure S2. Major and minor groups identified: maximum likelihood method based on the Hasegawa-Kishino-Yano model (Hasegawa et al., 1985). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 64 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 1,381 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 (Kumar et al., 2015).
Nexus files associated with analyses
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