Mating Season, Egg-Laying Season, and Internal Gametic Association in the Sympatrically Occurring Fluffy Sculpin (Oligocottus snyderi) and Rosy Sculpin (O. rubellio)
Takeshi Ito, Breana N. Goldman, Satoshi Awata, and Karen D. Crow
Ichthyology & Herpetology 112: 10–20
Fig. S1. Comparison of anal fin modification between Fluffy Sculpin (Oligocottus snyderi) and Rosy Sculpin (O. rubellio). (A) Male Fluffy Sculpin, with fin membrane thickening concentrated in first anal ray to the tip. (B) Male Rosy Sculpin, with fin membrane thickening concentrated in first and second (partial) anal ray to the tip. (C) Female Fluffy Sculpin, with no elongated anal fin. (D) Female Rosy Sculpin, with no elongated anal fin.
Fig. S2. Confirmation of species identity using a gene tree constructed from 561 bp CO1 sequences of ten species including marine sculpins (Psychrolutidae). Gene tree topologies were constructed using MEGA version X, with the maximum likelihood method with GTR+G+I model and 1,000 bootstrap replicates. The branch labels indicate bootstrap values. The samples collected in the present study are shown using the code Ala, Can, Oru, and Osn for fish identified as Smoothhead Sculpin (Artedius lateralis), Wooly Sculpin (Clinocottus analis), Fluffy Sculpin (Oligocottus snyderi), and Rosy Sculpin (O. rubellio), respectively. Scientific names with accession numbers were downloaded from NCBI, and Scorpaenichthys marmoratus (EU403072) was used as the outgroup in this tree. The * denotes the species that were misidentified in the field, while the ** represents the samples that had been misidentified and registered in GenBank.
Fig. S3. Confirmation of species identity using a gene tree constructed from 1051 bp Cytb sequences of ten species including marine sculpins (Psychrolutidae). Gene tree topologies were constructed using MEGA version X, with the maximum likelihood method with GTR+G+I model and 1,000 bootstrap replicates. The branch labels indicate bootstrap values. The samples collected in the present study are shown using the code Osn and Oru for fish identified as Fluffy Sculpin (Oligocottus snyderi) and Rosy Sculpin (O. rubellio), respectively. Scientific names with accession numbers were downloaded from NCBI, and Scorpaenichthys marmoratus was used as the outgroup in this tree.