Blackburn et al—Phylogeny of African Long-fingered Frogs (Arthroleptidae: Cardioglossa)

Phylogeny of African Long-Fingered Frogs (Arthroleptidae: Cardioglossa) Reveals Recent Allopatric Divergences in Coloration

David C. Blackburn, Stuart V. Nielsen, Sonia L. Ghose, Marius Burger, LeGrand Nono Gonwouo, Eli Greenbaum, Václav Gvoždík, Mareike Hirschfeld, Marcel T. Kouete, Chifundera Kusamba, Dwight Lawson, Patrick J. McLaughlin, Ange-Ghislain Zassi-Boulou, and Mark-Oliver Rödel

Ichthyology & Herpetology 109: 728–742

Supplemental Figure 1. Multilocus phylogeny of Cardioglossa based on the concatenated, partitioned, nuclear gene dataset. The backbone is from the RAxML analysis, although the MrBayes analysis produced a nearly identical topology. A black dot at each node indicates high support (e.g., Bayesian posterior probability >0.95, maximum likelihood bootstrap >70).

Supplemental Table 1. Sampling information including collection/voucher IDs, collection localities with approximate GPS coordinates, and GenBank accession details for all the samples included in our analyses.

Supplemental Table 2. List of PCR primers used in this study.

Supplemental Table 3. Uncorrected pairwise 12S/16S sequence differences (12S below the diagonal; 16S above) averaged over all sequence pairs between currently recognized species of Cardioglossa conducted in MEGA.

Best partitioning scheme from PartitionFinder

Chronogram from BEAST based on the concatenated dataset of mitochondrial and nuclear data

Consensus phylogeny based on MrBayes analysis of partitioned concatenated dataset of mitochondrial and nuclear data

Maximum likelihood phylogeny from RAxML analysis of partitioned concatenated dataset of mitochondrial and nuclear data

Nexus file of partitioned concatenated dataset of mitochondrial and nuclear data

Partitioning scheme implemented for RAxML analysis

Phylip file of concatenated dataset of mitochondrial and nuclear data